Skip to main content

A Comparison of Spectrophotometric and Oxidoreduction Potential Method for Laccase Activity Measurement: Crimson Publishers

A Comparison of Spectrophotometric and Oxidoreduction Potential Method for Laccase Activity Measurement by Rana Mustafa in Research in Medical & Engineering Sciences

Polyphenol oxidases (PPOs) oxidize phenolic compounds forming quinones, which undergo non-enzymatic reactions resulting in colored compounds. We studied ferulic acid and catechol oxidation by Myceliophthora thermophila laccases using oxidoreduction potential (ORP or Eh) as a novel method to determine PPO activity compared to the traditional spectrophotometric method. Eh varied time-dependently on ferulic acid and catechol concentrations. Eh increased to a maximum value (Ehmax), then decreased irreversibly proportional with substrate concentration. The ΔEh curves of ferulic acid and catechol oxidation at different concentrations by laccase exhibited two steps substrate oxidation: enzymatic and non-enzymatic; linear segments with increasing and decreasing slopes (rate of ΔEh change). The Km values determined spectrophotometrically and ORP were 0.55, 0.75 and 0.39, 0.32mM for catechol and ferulic acid, respectively. The kinetic parameters (Km, Vmax) of enzymatic reaction were not significantly different between the spectrophotometry and ORP methods. The ORP method is simple, low-cost and fast enabling the differentiation between the enzymatic and nonenzymatic phenol oxidation reactions.



For more open access journals in crimson publishers 
Please click on link: https://crimsonpublishers.com
Please click on link: https://crimsonpublishers.com/rmes/ 
Labels:

Comments

Post a Comment

Popular posts from this blog

The Malachite Wen-Myeloid Sarcoma- Crimson Publishers

The Malachite Wen-Myeloid Sarcoma- Crimson Publishers   Contingent to World Health Organization (WHO) classification, myeloid sarcoma is denominated as a tumefaction comprised of myeloblasts amalgamated at an anatomical site divergent from the bone marrow which engenders distortion of normal tissue architecture. Generally, myeloid sarcoma is contemplated to be equivalent to and concurrent with acute myeloid leukaemia (AML), although lesions may occur in isolation. Additionally designated as granulocytic sarcoma, chloroma or extramedullary myeloid tumour, myeloid sarcoma categorically represents as a distinct tumefaction associated with architectural effacement of circumscribing soft tissue. Myeloid sarcoma may emerge as a de novo lesion or manifest as therapy related myeloid neoplasm or as disease progression within myeloproliferative neoplasm (MPN), myelodysplastic syndrome (MDS) or myelodysplastic / myeloproliferative neoplasm (MDS / MPN). In contrast, leukaemia cutis (LC) is...
Post a Comment
Read more

Use of Thymbra Species Spreading in the Flora of Turkey for Medicinal Purposes- Crimson Publishers

Use of Thymbra Species Spreading in the Flora of Turkey for Medicinal Purposes- Crimson Publishers In terms of richness of plant species, Turkey has an important position in the world. It contains about 9,000 plant species and 11,500 taxa. Moreover, more than 1,000 plant species are used as medicinal and aromatic plants in our country. Medicinal plants are mostly evaluated by gathering from nature. Lamiaceae family covers many well-known and economically important medicinal types such as mint, sage, balm, thyme, lavender, basil. These plants, with wide adaptation limits, are well adapted to temperate and tropical regions. Although it is found in the natural flora of almost every region in our country, the Aegean and Mediterranean regions have an important position in terms of endemic species of the family. Although there are many aromatic plant species belonging to the family Lamiaceae described as thyme in Turkey, especially species containing the essential oil carvacrol and thymol ...
Post a Comment
Read more

Conservative Respiratory Therapeutic Management of COVID-19- Crimson Publishers

Conservative Respiratory Therapeutic Management of COVID-19- Crimson Publishers   COVID-19 symptoms can linger for weeks or even months after the initial infection. This phenomenon has been dubbed “long covid” by some. Because the coronavirus primarily affects the lungs, one of the long COVID-19 breathing symptoms after initial recovery can be ongoing breathlessness. The key symptoms of COVID-19 virus are cough, fever, breathlessness, anxiety, delirium, and agitation, but you may also have fatigue, muscle ache and headache [1]. Please note that severe breathlessness may cause anxiety, which may increase the breathlessness further. This leaflet has some simple breathing exercises you can do to help relieve the symptoms of breathlessness, Depression, and anxiety if you are self-isolating at home. The following tips should help you to manage breathlessness, Depression, and anxiety. https://crimsonpublishers.com/rmes/fulltext/RMES.000712.php For more open access journals in crims...
Post a Comment
Read more